Figure 6. Drp1 rescues PINK1^KO induced crushed thorax and mitochondrial abnormality in dATG7‐deficient Drosophila .

• A, B
  Drp1 rescues PINK1^KO induced collapsed thorax phenotype in dATG7‐deficient Drosophila. Representative SEM images of thorax from PINK1^WT, (upper panels) and PINK1^KO (lower panels) flies combined with dATP7 deficiency (dATG7^KO) or their controls (dATG7^WT), followed by expressing either mhc‐gal4 (Mhc/+) alone, or Mhc‐gal4‐driven expression of human Drp1 (Mhc>hDrp1^WT), mhc‐gal4‐driven expression of human Drp1^S616A (Mhc>hDrp1^S616A), or mhc‐gal4‐driven expression of human Drp1^S616D (Mhc>hDrp1^S616D) are shown (A). Quantitation of crushed thorax in flies with indicated genotypes is presented (B). > 100 flies for each experiment are analyzed. Scale bar = 200 μm. One‐way ANOVA followed with Tukey's test. ***P < 0.001. Data were presented as mean ± SEM of three independent experiments.
• C, D
  Drp1 rescues PINK1^KO induced mitochondrial defects in dATG7^KO Drosophila. Representative TEM images of indirect flight muscle from PINK1^WT (upper panels) and PINK1^KO (lower panels) flies combined with dATG7^KO or their controls (dATG7^WT), followed by expressing either mhc‐gal4 (Mhc/+) alone, or Mhc‐gal4‐driven expression of human Drp1(Mhc>hDrp1^WT), mhc‐gal4‐driven expression of human Drp1^S616A(Mhc>hDrp1^S616A), or mhc‐gal4‐driven expression of human Drp1^S616D(Mhc>hDrp1^S616D) are shown (C). Scale bar = 1 μm. Normal and abnormal mitochondrial number per mm² from each experimental group were and quantified. > 3 flies/group and 3–6 pictures of different microscopic fields from each fly were analyzed per repeat. Bars represent the mean ± SEM of three independent experiments (D).
• E
  Drp1 rescues PINK1^KO induced ATP reduction in Drosophila. ATP contents of thorax muscle tissues from the indicated genotypes were measured and normalized against the protein levels One‐way ANOVA followed with Tukey's test. ***P < 0.001, ns: no significance. Data were presented as mean ± SEM of three independent experiments.