Figure EV3. CDC7 inhibition decreases colocalization of MRE11 with RPA2.

1. U2OS cells were treated with 4 mM HU in the presence or absence of 10 μM XL413 for 24 h. RPA2 (green) and MRE11 (red) were detected by immunofluorescence. Insets I–II represent enlargements of selected region of the merged images.
2. Quantification of RPA2 and MRE11 colocalization was assessed with ImageJ in ˜200 randomly selected cells for each condition from three biological replicates and expressed as Pearson's correlation coefficient. Error bars represent SEM. Statistical significance was assessed by Student's t‐test (**P ˂ 0.01).

Source data are available online for this figure.