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. 2020 Jun 21;10(2):e113. doi: 10.1002/ctm2.113

FIGURE 4.

FIGURE 4

HucMSC‐Ex inhibits neddylation process of FHC in inflammatory environment (A) CCK8 analysis of FHC proliferation, *P < 0.05; **P < 0.01; ***P < 0.001 by ANOVA; (B) Western blot analysis of FHC proliferation; (C) Western blot analysis of neddylation‐related molecules in FHC; (D) Co‐IP analysis of binding of NEDD8 to cullin 1; (E) QRT‐PCR analysis of neddylation‐related molecules expression in FHC, *P < 0.05; **P < 0.01; ***P < 0.001 by ANOVA ; (F) IF analysis of expression of neddylation‐related molecules in FHC (cullin 1) (scale bar = 50 μm); (G) IF analysis of neddylation‐related molecules in FHC (NEDD8) (scale bar = 50 μm); (H) IF analysis of P‐NF‐κB in FHC (scale bar = 50 μm)