FIGURE 4.

IRE increased the infiltration of memory CD4⁺ and effector CD8⁺ T cells in pancreatic tumor tissue. A, Analysis of T cells isolated from tumor, spleen, and lymph node and the comparisons of associated ratios of memory and effector CD4⁺ T cells in subcutaneous tumor models before and after IRE treatment. Memory CD4⁺ T cells increased significantly after IRE in tumor and lymph node, while no significant differences were observed in other groups. B, Analysis of T cells isolated from tumor, spleen, and lymph node, and the comparisons of associated ratios of memory and effector CD8⁺ T cells in subcutaneous tumor models before and after IRE treatment. Memory CD8⁺ T cells in spleen, lymph node, and effector CD8⁺ T cells in tumor increased significantly after IRE. Two‐tailed Student's t‐test was performed. *P < .05, **P < .01, ***P < .001, NS, not significant