IRE enhanced the immune memory of T cells. A, Experimental protocol for the evaluation of the abscopal effect of ablation in suit by IRE. B, The comparisons of subcutaneous tumor growth curves and survival curves after the ablation by IRE in orthotopic tumor model. IRE significantly suppressed tumor growth and extended survival, compared with sham‐operation and no treatment control. C, Experimental protocol for the evaluation of the abscopal effect of tumor culture supernatants (TSN) of Pan02 3 days after electroporation with different strength levels. D, The comparisons of first‐implanted and rechallenged tumor growth curves after exposure to the TSN of Pan02 3 days after electroporation with different strength levels. Original tumors were not observed in mice, which were first implanted with TSN of Pan02 after electroporation. Additionally, the growth of rechallenged tumors decreased along with the initial treatment with TSN of Pan02 3 days after electroporation with different strength levels. E, The proportion of infiltrated CD4+ and CD8+ T cells in lymph node of mice after exposure to TSN of Pan02 3 days after electroporation with different strength levels. No significant changes were observed for CD4+ T cell, while the ratios of CD8+ T cell increased significantly along with the increasing levels of strength of electroporation. F, The proportion of infiltrated CD4+ and CD8+ T cells in spleen of mice after exposure to TSN of Pan02 3 days after electroporation with different strength levels. No significant changes were observed for CD4+ T cell while the ratios of CD8+ T cell increased significantly along with the increasing levels of strength of electroporation. Log‐rank test and one‐way analysis of variance (ANOVA) with Bonferroni comparison test were performed. P < .05, **P < .01, ***P < .001, NS, not significant