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. 2020 May 25;21(8):e50348. doi: 10.15252/embr.202050348

Figure 2. Infected macrophages can internalize and deliver degraded extracellular macromolecules to intracellular S. aureus .

Figure 2

  1. Internalization and processing of DQ™ Green BSA by uninfected and S. aureus (in blue)‐infected RAW 264.7 macrophages at 10 h post‐infection. These images are representative of three independent experiments.
  2. Quantitation of DQ‐BSA green fluorescence displayed by either uninfected or S. aureus (in blue)‐infected RAW 264.7. Shown is the mean fluorescence intensity (MFI) in arbitrary units (a.u) ± standard deviation where each symbol represents the measurement of a single cell. The data are derived from three independent experiments, and n.s. indicates the data are not significant as determined by an unpaired two‐tailed t‐test (P > 0.05).
  3. Fluorescence‐based proliferation assays were performed in conjunction with the probe DQ™ Green BSA. Processed DQ™ Green BSA could be found co‐localizing with intracellular mCherry‐expressing S. aureus (in red) and with intracellular S. aureus that has replicated (i.e., proliferation dye negative) at 10 h post‐infection as depicted here. Data information: Scale bars equal 10 μm.