Figure EV5. Features of neutrophil counts in peripheral blood, transmigration ability and cytokine expression in neutrophils from Lmnb1^TG vs WT mice.

• A
  Neutrophil counts in peripheral blood from Lmnb1^TG mice and their WT littermates (n = 4 biological replicates).
• B
  Relative transmigration was measured using Boyden chamber with pore size of 3.0 μm. The pore transmigration ability in neutrophils from the Lmnb1^TG mice was expressed as relative transmigration compared to that in neutrophils from WT littermates (n = 3 biological replicates).
• C, D
  The bone marrow neutrophils from Lmnb1^TG mice and their WT littermates were treated with 10 μM PAF for 16 h and then fixed with 2% PFA and permeabilized with 0.1% Triton X‐100, following immunocytochemistry staining. The neutrophil surface marker Ly6G was stained by PE‐conjugated rat anti‐mouse Ly6G Ab. FITC‐labeled rat anti‐mouse IL‐17A antibody or FITC‐labeled rat anti‐mouse TNF‐α antibody was used to detect IL‐17A or TNF‐α. DNA was stained by DAPI for or panels (C, D).

Data information: Panels (A, B) are summary analysis that was calculated based on neutrophil counts of the blood slides with May–Grünwald–Giemsa staining (A), the arbitrary fluorescent unit of SYTOX Green staining of the lysed neutrophils that transmigrated to the bottom chamber of the 24‐well transwell plates, from at least 3 independent biological replicates (B). The differences between groups P > 0.05 are indicated as no significant difference (NS). Data are given as mean ± SD. Comparisons were calculated by the Student t test.