Figure 7.

SNPs12/13 explain additional variation in plate count and D‐V neuromast patterning of Lake Washington stickleback after accounting for Cnv770. Adjusted left plate count, D‐V neuromast pattern, and Mp neuromasts per segment are plotted for Lake Washington fish by both genotype at Cnv770 (the marker with the highest single LOD score in the association mapping) and SNPs12/13, which controlled additional variation in plate count in the Puget Sound crosses (Fig. 3C). Mean trait value ± SD are depicted by black lines and whiskers. Fish that are heterozygous at both Cnv770 and SNPs12/13 have significantly more plates than fish heterozygous at Cnv770 and homozygous LL at SNPs12/13 (t ₁₂ = 4.4, P = 0.00085) and differ in D‐V neuromast patterning (t ₁₂ = 2.7, P = 0.03). The trait values plotted are adjusted for sex, standard length, and collection, and therefore vary outside the normal range of values. There was a single fish with a historical recombination event on both chromosomes between Cnv770 and SNPs12/13, resulting in an LL genotype at Cnv770 and a CC genotype at SNPs12/13. Significance levels: ^{* * *} P < 0.001; ^{* *} P < 0.01; ^* P < 0.05; P > 0.05 (not significant).