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. 2020 Jun 17;21(8):e49956. doi: 10.15252/embr.201949956

Figure EV4. Role of Arg‐171 of mouse PABPN1L in RNA binding.

Figure EV4

  • A
    A table showing the Tm in Fig 6A–C.
  • B
    Sequence alignment of mouse PABPN1L (MusPABPN1L) and Citrus sinensis PABPN1(CsPABPN1). The broken circle and arrowhead indicate Arg‐171 of mouse PABPN1L.
  • C
    Sequence alignment and a phylogenetic tree analysis of PABPN1L. PABPN1L aminol acid sequences from H. sapiens (A6NDY0), M. musculus (Q5XFR0), R. norvegicus (B0BNE4), G. gallus (F1NWW9), X. tropicalis (Q28GL9), and D. rerio (E9QGY6) were analyzed by Clustal Omega Multiple Sequence Alignment algorithm (https://www.ebi.ac.uk/Tools/msa/clustalo/). The phylogenetic tree was plotted based on results from sequence alignments using the neighbor‐joining tree algorithm without distance corrections. Sequence alignments and quality parameters of RNA recognition motif (RRM, highlighted in light yellow) and nucleus localization signal (NLS, highlighted in light blue) were generated by Jalview (http://www.jalview.org/). The broken circle and arrowhead indicate Arg‐171 of mouse PABPN1L.