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. 2020 Aug 4;10:13136. doi: 10.1038/s41598-020-68515-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased number of glomerular apoptotic cells in diabetic Alk1ΔEC +/− mice. (A) Immunofluorescence analysis of cleaved caspase 3 (green) in non-diabetic or diabetic C5Cre and diabetic Alk1ΔEC +/− mice. (B) Quantification was performed by evaluating the number of positive cells per glomerular nuclei. (C) TUNEL and WT1 immunofluorescent staining of non-diabetic or diabetic C5Cre or Alk1ΔEC +/− mice. (D) Cleaved-caspase 3 and PDGFRB immunostaining of non-diabetic or diabetic C5Cre and Alk1ΔEC +/− mice. (E) Quantification was performed by evaluating the number of double-positive cells per glomerular nuclei. Analysis show quantification of at least 50 glomeruli from 5 mice/group. **p < 0.01. Scale bars: 60 µm.