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. 2020 Aug 4;10:13136. doi: 10.1038/s41598-020-68515-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Alk1ΔEC homozygote deletion on renal function. (A) Immunofluorescent staining of Alk1 in kidney sections of C5Cre and Alk1ΔEC−/− mice seven days after tamoxifen delivery. (B) Immunoblot of Alk1 expression in isolated glomeruli of C5Cre and Alk1ΔEC−/− mice after tamoxifen injections. Urine albumin (C), serum creatinine (D), glomerular filtration rate (E), serum and urine glucose (F) in C5Cre and Alk1ΔEC−/− mice 7 days following tamoxifen injections. (n = 8 mice/group). Scale bar: 50 µm.