FIG 2.

Expression and purification of UL11 constructs. (A) Schematic representation of UL11 sequence and UL11 constructs used in this work. Protease recognition sites are shown in gray. Construct names and molecular masses calculated from the sequence (MM_Calc) and determined experimentally (this paper) (by SEC, MS, and SEC-SAXS) are shown. WT, wild type; H₆, His₆ tag; GST, glutathione S-transferase tag; StII, Strep-tag II. (B) Purification scheme for UL11 constructs. (C) Coomassie blue-stained gels of purified UL11 constructs.