FIG 5.

UL11 undergoes liquid-liquid phase separation. (A) Microscopic images of UL11 constructs in solution show representative images of liquid-liquid phase separation (LLPS) seen under a variety of conditions, including purification buffer and for H₆-UL11, crystallization screens (1:1 mix of protein-solution in vapor diffusion chamber). (B) Macroscopic images of UL11-StII in solution in purification buffer show reversibility of UL11-StII phase separation (cloudiness) upon temperature cycling. (C) Time lapse microscopic images of UL11-StII condensates in purification buffer display hallmark liquid-like properties of surface wetting and droplet merging (selected example in orange box). (D) LLPS (separation, orange circles; clear solution, blue triangles) occurs with increasing protein concentration, increasing salt concentration, and increasing molecular crowding.