TABLE 2.
Parameter | Value(s) for UL11-StII |
---|---|
Guinier analysis | |
I(0) (cm−1)a | 0.00477 ± 0.000041 |
Rg (Å)b | 24.29 ± 2.0 |
qmin (Å−1)c | 0.01254 |
qRg maxd | 1.30239 |
Coefficient of correlation, R2e | 0.938 |
P(r) analysis (forced to 0/not forced)f |
|
I(0) (cm−1) | 0.004748 ± 0.00003566/0.004866 ± 0.00009504 |
Rg (Å) | 24.74 ± 0.1599/26.29 ± 1.712 |
Dmax (Å)g | 76/120 |
q range (Å−1)h | 0.0074 − 0.2802/0.0074 − 0.2802 |
χ2 (total estimate from GNOM)i | 1.0385 (0.8640)/0.9935 (0.7203) |
AMBIMETER analysis of P(r) (forced to 0/not forced)f |
|
Compatible shape categoriesj | 720/153 |
Ambiguity scorek | 2.857/2.185 |
Uniquenessl | Highly ambiguous/might be ambiguous |
I(0), scattering intensity at zero angle.
Rg, radius of gyration.
qmin, lowest q value used in the Guinier analysis.
qRg max, maximum q value used in the Guinier analysis × Rg.
R2, linear fit of the Guinier plot.
(forced to 0/not forced) = P(r) forced to 0 at Dmax in DATGNOM (91)/P(r) not forced to 0.
Dmax, maximum dimension calculated from P(r).
q range, q values used in P(r) plot.
χ2 fit of P(r) to data. The total quality estimate from GNOM (91) is shown in the parentheses.
The number of shape topologies, M, compatible with P(r) evaluated by AMBIMETER (94).
The ambiguity score is log M and correlates with probability of finding a false-positive result in a three-dimensional (3D) reconstruction (94).
Uniqueness of 3D reconstruction of P(r) evaluated by AMBIMETER (94).