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. 2020 May 5;11(3):e00810-20. doi: 10.1128/mBio.00810-20

TABLE 2.

SAXS structural parameters

Parameter Value(s) for UL11-StII
Guinier analysis
    I(0) (cm−1)a 0.00477 ± 0.000041
    Rg (Å)b 24.29 ± 2.0
    qmin−1)c 0.01254
    qRg maxd 1.30239
    Coefficient of correlation, R2e 0.938
P(r) analysis
(forced to 0/not forced)f
    I(0) (cm−1) 0.004748 ± 0.00003566/0.004866 ± 0.00009504
    Rg (Å) 24.74 ± 0.1599/26.29 ± 1.712
    Dmax (Å)g 76/120
    q range (Å−1)h 0.0074 − 0.2802/0.0074 − 0.2802
    χ2 (total estimate from GNOM)i 1.0385 (0.8640)/0.9935 (0.7203)
AMBIMETER analysis of P(r)
(forced to 0/not forced)f
    Compatible shape categoriesj 720/153
    Ambiguity scorek 2.857/2.185
    Uniquenessl Highly ambiguous/might be ambiguous
a

I(0), scattering intensity at zero angle.

b

Rg, radius of gyration.

c

qmin, lowest q value used in the Guinier analysis.

d

qRg max, maximum q value used in the Guinier analysis × Rg.

e

R2, linear fit of the Guinier plot.

f

(forced to 0/not forced) = P(r) forced to 0 at Dmax in DATGNOM (91)/P(r) not forced to 0.

g

Dmax, maximum dimension calculated from P(r).

h

q range, q values used in P(r) plot.

i

χ2 fit of P(r) to data. The total quality estimate from GNOM (91) is shown in the parentheses.

j

The number of shape topologies, M, compatible with P(r) evaluated by AMBIMETER (94).

k

The ambiguity score is log M and correlates with probability of finding a false-positive result in a three-dimensional (3D) reconstruction (94).

l

Uniqueness of 3D reconstruction of P(r) evaluated by AMBIMETER (94).