Figure 3.

LGR6 Increases the Stemness and Promotes Metastasis in TNBC

(A) LGR6 increased the sphere-forming ability in LGR6-MDA-MB-468 cells and decreased it in LGR6 silenced BT549 cells (∗p < 0.05, ∗∗p < 0.01). (B) Transwell invasion assay demonstrating that LGR6 promotes cell invasion. Representative images of invaded cells are shown in the left panel, and the results are summarized in the right panel. The results are expressed as the mean ± standard deviation of three independent experiments (∗p < 0.05, ∗∗p < 0.01). (C) Representative images of H&E-stained sections derived from metastatic nodules at the lung surface. Original magnification, ×100. The micrometastases in the H&E-stained sections of individual mice were calculated. Each group had 5 mice. Bars, 50 μm. All data are shown as mean ± standard deviation (∗∗p < 0.01).