LGR6 Regulates the Wnt/β-Catenin Pathway in TNBC
(A) The protein expression of LGR6, β-catenin, BRCA1, Dnmt1, Dnmt3a, Dnmt3b, and PTEN in LGR6-MDA-MB-468 and control cells and between shLGR6-BT549 and control cells detected by western blot. β-actin was used as the loading control. The band intensities are quantified and normalized to β-actin intensities (∗p < 0.05, ∗∗p < 0.01). (B) Immunohistochemistry assay of BRCA1, Dnmt1, Dnmt3a, Dnmt3b, and PTEN expressions in shLGR6-BT549 and control cell xenografts.