Figure 1.

Evaluation of Micro-dystrophin Expression by Immunostaining Using a Human Dystrophin-Specific Antibody

(A and B) Representative photomicrographs of dystrophin immunostaining in the (A) quadriceps and (B) heart. TT, AAV made with the transient transfection method; HSV, AAV made with the herpes simplex virus system. Cytosolic staining in the mdx4cv image and low dose images in panel A is due to cross-reaction of the secdary antibody with immunoglobulin infiltrated in damaged muscle cells