Figure 3.

Evaluation of Micro-dystrophin Protein Expression by Western Blot

(A) Representative dystrophin western blot images showing abundant micro-dystrophin at the expected size in the quadriceps and heart of the treated mdx4cv mice. Vinculin was used as the load control. Fl-Dys, full-length dystrophin; μDys, micro-dystrophin. (B) LI-COR densitometry quantification of the intensity of the dystrophin band. ∗Significantly different from other dose groups. TT, AAV made with the transient transfection method; HSV, AAV made with the herpes simplex virus system. Please note that a nonspecific band was detected in heart western blot due to cross-reaction of the dystrophin antibody with an unknown protein in the mouse heart lysate. Data are presented as mean ± standard error of the mean. The difference was considered significant when p < 0.05.