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. 2020 Aug 4;40(8):BSR20201495. doi: 10.1042/BSR20201495

Figure 4. CEC of N-Hcy-HDL.

Figure 4

(A) N-homocysteinylation of HDL was confirmed by isoelectric focusing and Western blotting (1 μg TP/lane) after treatment with or without cysteamine. (B) The content of TC in intact HDL (Hcy(−)) and N-Hcy-HDL (Hcy(+)) were determined as ratios relative to TP content. (C–F) CEC was evaluated using 20 μg TP/ml or 1 mg TC/dl of each HDL subclass by ILG (C,E) and conventional (D,F) method. The results were indicated as the ratio to CEC of intact HDL. All samples were assayed in triplicate. Values are presented as means ± SD (n=4). The results were evaluated by paired t tests. Abbreviation: NS, no significance.