Figure 2.

Osteogenic differentiation potential of PL treated and untreated osteoblasts in vitro. FCS culture indicates cells expanded in standard culture medium. FCS + PL culture indicates cells expanded standard culture medium supplemented with PL. Cells from both culture conditions were transferred in standard osteogenic medium. (A) Alizarin Red staining, at weekly time intervals, for the two experimental culture groups transferred in osteogenic medium (osteo) or in standard culture medium (Ctrl). (B) Quantification of the Alizarin staining. The amount of staining present in each well was determined.