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. 2020 Jul 15;21(14):4980. doi: 10.3390/ijms21144980

Figure 2.

Figure 2

The transduction of 78 kDa glucose-regulated protein (GRP78) mitigates AngII-induced aggregate formation in VSMCs. (A–C) The rat aortic VSMCs infected with adenovirus encoding GRP78 or control green fluorescent protein (GFP) (100 multiplicity of infection (moi)) for 48 h were stimulated with 100 nM AngII (AII) for 48 h. Representative Proteostat staining images are shown. The scale bar indicates 10 μm. (A). The aggregate positive area per cell (B) and total attached cells (C) in HPF were evaluated with ImageJ software. The bars in the graphs show the mean ± SD from 3 independent experiments. ** indicates p < 0.01. *** indicates p < 0.001.