Table 3.

Immune solid-phase allergen chip (ISAC), ImmunoCAP and skin-prick testing (SPT): The advantages and disadvantages (table adapted from the World Allergy Organization GA² LEN consensus document [76]).

	Advantages	Disadvantages
ISAC	No interference due to high total IgE Less allergen needed per assay 112 allergens can be analysed in parallel Natural and recombinant proteins are compatible 30 μL of serum or plasma	Manual method Less sensitive Not recommended for monitoring sensitization May cause interference between IgE and IgG Semi-quantitative assay
ImmunoCAP	Appropriate for monitoring sensitization High sensitivity Quantitative analysis Natural and recombinant proteins, as well as crude extracts, can be applied	40 μL of serum needed per allergen One allergen per assay Detects low-affinity antibodies which may not be clinically relevant
Skin Prick Test (SPT)	Prompt readings Extract-dependent high sensitivity	One allergen per prick Only crude extracts used Manual Cannot be used for monitoring sensitization