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. 2020 Jul 19;21(14):5104. doi: 10.3390/ijms21145104

Figure 6.

Figure 6

GLUT1 promoted cellular glucose uptake and NLRP3 inflammasome activation. (A) Immunoblot of GLUT1 in THP-1-derived macrophages treated with GLUT1 small interfering RNA (siRNA) or control siRNA. (B) Flow cytometry plots of three experiments showing glucose uptake in WT and Cbl-KO THP-1-derived macrophages treated with GLUT1 siRNA or control siRNA. (C) Immunoblot analysis of caspase-1 (p20) and IL-1β (p17) in culture supernatants of WT and Cbl-KO THP-1-derived macrophages treated with GLUT1 siRNA or control siRNA and then treated with nigericin. (D) Enzyme-linked immunosorbent assay of IL-1β in the supernatants of WT and Cbl-KO THP-1-derived macrophages treated with GLUT1 siRNA or control siRNA and then treated with nigericin. * p < 0.05; ** p < 0.01. All results are presented as the mean ± SD of the three independent experiments and were analyzed using Student’s t-test.