Figure 3.

Reduction of the anti-C. acnes dose of clindamycin in the presence of fermentation media. The ears of ICR mice were intradermally injected with C. acnes (10⁷ CFU), plus 0.1 or 1% of clindamycin (CDM) or H₂O, along with media collected from culture of S. epidermidis (S. epi), with or without 2% PEG-8 Laurate. (a) Ears thickness (mm) was measured for 6 days after injection. (b) The CFUs were counted after plating serial dilution (1:10⁰–1:10⁵) of ear homogenates agar plates. (c) The levels of MIP-2 cytokines in ear homogenates were quantified by ELISA. Data are represented as mean ± SD of three independent experiments using three mice per group. ** p < 0.05; *** p < 0.001 (two-tailed t-tests); ns = non-significant.