Figure 4.

Osteoclastogenic potential of the alveolar bone cells and the long bone cells in the basic medium and the basic medium supplemented with vitD₃. Osteoclastogenic potential of the co-cultures with the PBMCs and long bone cells and the PBMCS and alveolar bone cells. Graphs depict the basic medium and the basic medium + vitD₃. (A) The total number of osteoclasts induced by athe lveolar bone cells, long bone cells, and the PBMCs alone at 21 days. Differentiation was further assessed with qPCR for osteoclast-specific genes (B,C). (B) The relative expression of DC-STAMP. (C) Relative expression of TRAcP. Significance for the number of osteoclasts was measured using a one-way ANOVA with a Tukey post-hoc test and the significance of the relative gene expression was measured using 2-way ANOVA with Bonferroni post-tests. * p < 0.05.