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. 2020 Jul 17;21(14):5072. doi: 10.3390/ijms21145072

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Differences in the proteins and the genes involved with osteoclastogenic differentiation in the alveolar bone cells and the long bone cultures. Graphs depict the basic medium and the basic medium + vitD₃ (A) Concentration of TNF-α in media at day 7. (B) Relative expression of IL-6. (C) Relative expression of RankL. (D) Relative expression of osteoprotegerin (OPG). (E) The ratio RankL/OPG gives an indication for osteoclast activation. (F) Relative expression of COX2. Significance was measured using a 2-way ANOVA with Bonferroni post-tests. * p < 0.05.

Differences in the proteins and the genes involved with osteoclastogenic differentiation in the alveolar bone cells and the long bone cultures. Graphs depict the basic medium and the basic medium + vitD₃ (A) Concentration of TNF-α in media at day 7. (B) Relative expression of IL-6. (C) Relative expression of RankL. (D) Relative expression of osteoprotegerin (OPG). (E) The ratio RankL/OPG gives an indication for osteoclast activation. (F) Relative expression of COX2. Significance was measured using a 2-way ANOVA with Bonferroni post-tests. * p < 0.05.