Table 2.
Epidemiological data on toxoplasmosis-like abortion outbreaks in sheep flocks in Spain (2015–2018): etiology confirmation and T. gondii isolation
| Sample ID | T. gondii-associated abortion outbreak ID | Municipality (province) | Descriptiona | Period of time (lambing season) | Samples collectedb | Isolate designation (original sample) | RFLP genotype ID# (ToxoDB) | ||
|---|---|---|---|---|---|---|---|---|---|
| Foetal brain tissues | Placental tissues | Lamb brain tissues | |||||||
| 15/121 | 1 | Fuentes de Valdepero (Palencia, North) | Assaf, 1450, 20% | 2015/2016 | 3 (3, 2, 1) | 2 (2, 2, 0) | – | TgShSp1 (15/121.4)c | #3 |
| 15/141 | 2 | Artajona (Navarra, North) | Assaf, 3800, 10% | 2015/2016 | 2 (2, 1, 1) | – | – | TgShSp2 (15/141.2) | #1 |
| 17/4, 17, 18, 19, 21, 24, 28 | 3 | Autillo de Campos (Palencia, North) | Assaf, 2800, 4.3% | 2016/2017 | 24 (22, 6, 6) | 6 (6, 3, 1) | 7 (1, 1, 0) | TgShSp3 (17/4.1); TgShSp4 (17/21.2); TgShSp5 (17/28.1); TgShSp6 (17/19.3) | #3 |
| 17/61 | – | Villaconancio (Palencia, North) | Assaf, 600, 5%, Border disease virus | 2016/2017 | 2 (0, 0, 1) | – | – | – | |
| 17/5, 15 | 4d | Benavente (Zamora, North West) | Assaf, 500, 10% | 2016/2017 | 3 (2, 2, 1) | 3 (2, 0, 0) | – | – | |
| 17/6, 20, 22, 23, 25, 27, 41 | 5d | Mayorga (Valladolid, North) | Assaf, 1600, 10% | 2016/2017 | 11 (5, 2, 2) | 4 (2, 0, 2) | – | – | |
| 17/94, 17 2/7, 17 2/6, 17 2/8, 17/29, 17/32, 17/36, 17/52 17/62 | 6d | Villamañán (León, North-West) | Assaf, NA | 2016/2017 | 14 (6, 4, 2) | 7 (3, 0, 0) | 5 (0, 0, 0) | – | |
| 17/42 | – | Alcarraz (Lérida, North-East) | Lacaune, 800, 25% | 2016/2017 | 4 (0, 0, 0) | 1 (0, 0, 0) | – | – | |
| 17/49 | – | Fuentes de Valdepero (Palencia, North East) | Assaf, 1600, 5% | 2016/2017 | 2 (0, 0, 0) | 1 (0, 0) | – | – | |
| 17/220, 221, 222, 223, 224, 225, 18/1, 4, 10 | 7 | Navas de Oro (Segovia, Centre) | Merino, 900, 83% | 2017/2018 | 12 (10, 5, 2) | 6 (6, 1, 0) | 3 (1, 1, 0) | TgShSp7 (18/4.2) | #3 |
| 17/227 | – | Santa Cruz de Mudela (Ciudad Real, Centre) | Manchega, 1200, 5% | 2017/2018 | 3 (0,0,0) | – | – | – | |
| 18/3 | – | Benavente (Zamora, North–West) | Assaf, 500, 15%, Neospora caninum | 2017/2018 | 1 (0, 0, 0) | 1 (0, 0, 0) | – | – | |
| 18/12 | – | Benegiles (Zamora, North–West) | Assaf, 1049, 15.3%, Neospora caninum | 2017/2018 | 2 (0, 1, 0) | – | – | – | |
| 18/5, 17 | – | Casas de Juan Núñez (Albacete, East) | Manchega, 3000, 40% | 2017/2018 | 6 (0, 0, 0) | 1 (0, 0, 0) | – | – | |
| 18/6 | – | Ledesma (Salamanca, West) | Castellana, 20, 25%, Neospora caninum | 2017/2018 | 1(0, 1, 0) | 1 (0, 0, 0) | – | – | |
| 18/7 | 8 | Catadau (Valencia, East) | Lacaune, 2400, 21% | 2017/2018 | 2 (2, 2, 0) | – | TgShSp8 (18/7.2) | #3 | |
| 18/14, 15, 16, 18, 20 | 9 | Cuevas de Almudén (Teruel, East) | Lacaune, 700, 50% | 2017/2018 | 62 (50, 29, 18) | 6 (6, 5, 1) | 3 (0,0,0) | TgShSp9 (18/18.1); TgShSp10 (18/15.1); TgShSp18 (18/16.1) | #3 |
| 18/23 | – | Moral de la Reina (Valladolid, North) | Assaf, 3000, 15% | 2017/2018 | 5 (0, 0, 0) | 1 (0, 0, 0) | – | – | |
| 18/36, 38 | – | Valdetorres (Badajoz, West) | Merino, 450, 20%, Neospora caninum | 2017/2018 | 3 (0, 1, 1) | – | – | – | |
| 18/21 | – | Autillo de Campos (Palencia, North) | Assaf, 3000, > 1% | 2017/2018 | 5 (0, 0, 0) | – | – | – | |
| 18/219, 228 | 10d | Villafrechos (Valladolid, North) | Assaf, 1100, 12% | 2017/2018 | 4 (2, 2, 0) | – | – | – | |
| 18/222, 226 | 11d | Aguilar de Campos (Valladolid, North) | Lacaune, 2500, 25%, Coxiella burnetii | 2017/2018 | 11 (11, 2, 4) | 2 (2, 0, 0) | – | – | |
| Total | 11 | 182 (115, 59, 3, 9) | 42 (29, 11, 4) | 18 (2, 2, 0) | |||||
aBreed, flock size, % of abortion, other pathogens detected. Screening for common protozoal, bacterial and viral abortifacient agents was performed as reported in references [26] and [27]
bPCR-positive cases, cases with consistent lesions, cases with compatible lesions
cIsolation reported in reference [32]
dBioassay in mice was not successful or was not carried out due to previous tissue freezing
na, data not available