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. 2020 Jul 20;21(14):5121. doi: 10.3390/ijms21145121

Figure 1.

Figure 1

Aging aggravates insulin resistance and aged BAFF−/− (B-cell-activating factor) mice shows enhanced glucose tolerance despite increased body weight. Young and old mice were maintained on a normal chow diet for 2 months and 10 months, respectively. Changes of (A) body weight, (B) glucose tolerance and (C) insulin sensitivity were measured (n = 5). Mice fasted for 16 h, and the blood glucose levels were measured at 0, 15, 30, 60, 90 and 120 min after intraperitoneal injection of glucose (2 g/kg) or insulin (0.5 U/kg). BAFF−/− and C57BL/6J mice were maintained on a normal chow diet for 10 months. (D) Body weights of 2- and 10-month-old adult male mice (n = 4–12). (E) Tissue weights of 10-month-old mice (n = 9–12). (F) Hematoxylin and eosin (H&E) staining sections of adipose tissues. Adipose tissues were fixed in 10% v/v formalin/PBS, and then embedded in paraffin for staining with hematoxylin and eosin. Images were obtained under a microscope at a magnification of X100. (G and H) Glucose tolerance test and insulin tolerance test of 10-month-old male mice (n = 8–12). Data represent means ± SD. # p < 0.05 and ### p < 0.001 between young and old mice and * p < 0.05, ** p < 0.01, *** p < 0.001 between wild-type and BAFF−/− mice. SAT: subcutaneous adipose tissue, EAT: epididymal adipose tissue, BAT: interscapular brown adipose tissue.