Figure 3.

BAFF depletion enhances expression of genes involved in thermogenic program and alternative macrophage activation in subcutaneous adipose tissue. (A) Effect of BAFF deficiency on thermogenic program gene expression (n = 9–12). Gene expression level is normalized with mRNA expression level of Arbp. (B) Effect of BAFF deficiency on UCP1 protein expression level (n = 4–5). Proteins were extracted from the tissue for SDS-PAGE-immunoblot analysis. Effect of BAFF deficiency on the expression of genes involved in (C) M2-like macrophage activation, (D) mature eosinophil cell marker and (E) anti-inflammatory cytokine (n = 9–12). Data represent means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 between wild-type and BAFF^−/− mice.