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FLIM images of the released fluorophore after the fluorogenic reaction of GG-DNBS with biothiols in N2a cells without (a) and with the addition of Aβ42 aggregates, formed after incubating for 24 h (b) and 48 h (c,d). The pseudocolor scale represents the fluorescence lifetime of the probe, τprobe, between 0 and 4 ns.
