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. 2020 Jul 19;21(14):5100. doi: 10.3390/ijms21145100

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Clonogenic assay after different treatments of Panc1 tumor cells. Representative images of formalin-fixed, crystal violet stained colonies 8 days after treatment (A). Reliable counting of the colonies was based on dividing the plate areas into segments (B). Statistical analysis revealed a significant reduction after R+mEHT treatment compared to the control group (p = 0.0195) (C). Kruskal–Wallis test also showed a significant difference among the 4 groups (p = 0.0064) TER = 3.1. The asterisk (*) marks the value of p ≤ 0.05.