Figure 6.

Demonstration of digital image analysis using the cleaved caspase-3 immunoreaction in R+mEHT treated Panc1 culture as an example. The NuclearQuant module of the QuantCenter software allowed the segmentation and counting of brown immunopositive cells (A, scale bar: 50 µm) and to determine their proportion to the whole population stained using hematoxylin. The accurately selected masque highlights positive (yellow labels) and negative (blue labels) cells shown also at high power in insets (B, scale bar: 10 µm).