Figure 7.

(A) The mRNA expression of ATXN2L in human SH-SY5Y neuroblastoma cells was induced by nutrient deprivation in HBSS medium over 48 h with low glucose/no amino acids, in the absence of lipids normally supplied via FCS, similar to the expression of (B) ATXN2 mRNA. (C) For the induction peak at 24 h, the expression upregulation of ATXN2L was partially prevented upon supplementation of 10% FCS, while glucose or amino acids alone had no rescue effect; (D) FCS addition also diminished ATXN2 expression, while glucose and amino acid administration enhanced the expression further; (E) The HBSS induction of ATXN2L was reduced at least as much by low cholesterol concentrations as by FCS, while higher dosage of cholesterol elicited even stronger upregulations. (F) This effect was similarly observed for ATXN2. A-D: n = 3; E/F: n = 8. Statistical testing was done by one-way ANOVA, significance levels were illustrated by asterisks: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Comparison was always in respect to nutrient abundant control condition, if not stated otherwise.