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. 2020 Jul 19;21(14):5093. doi: 10.3390/ijms21145093

Figure 7.

Figure 7

Infiltration of macrophages in the cornea after alkali injury. Representative photomicrographs of infiltrating macrophages in the corneal limbus after instillation of the vehicle (a) and PPARα+γ (b) on day 1 showed prominent infiltration of ED-1-positive macrophages on day 1 in the vehicle group, but significantly suppressed infiltration in the PPARα+γ group (c). Black arrow indicates an ED-1-positive cell (a). PPARγ and PPARα+γ treatment significantly suppressed ED-1-positive macrophages on day 1 compared with vehicle treatment (c). Data are presented as the mean ± standard error (n = 8 samples/group). *p < 0.05. Although few ED-2-positive M2 macrophages were present in the peripheral cornea on day 4 after alkali burn in the vehicle group (d), PPARα+γ treatment upregulated M2 macrophages (e). Red arrows indicate ED-2-positive cells (d), (e). Bar chart of the number of ED-2-positive cells shows a statistically significant difference between the vehicle and PPARγ groups, similar to the PPARα+γ instillation group (f). Bar, 50 μm (a), (b), (d), (e). Data are presented as the mean ± standard error (n = 8 samples/group). **p < 0.01, *p < 0.05.