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. 2020 Jul 21;21(14):5147. doi: 10.3390/ijms21145147

Figure 1.

Figure 1

Nonexpressor of PR genes 1 (NPR1) inhibited Psm ES4326/AvrRpt2 (AvrRpt2)-induced autophagy. (A) Autophagosomes formation. GFP-ATG8a and npr1 (GFP-ATG8a) treated with four groups: MgCl2; concanamycin A (ConA); ConA + AvrRpt2 and ConA + wortmannin (WM) + AvrRpt2 and then examined by confocal microscopy. Scale bars, 20 µm. Numbers of puncta per section in the root cells of GFP-ATG8a or npr1 (GFP-ATG8a) seedlings in the left. n = 10 sections from three independent experiments per genotype. (B) Western Blot to detect autophagy flow in GFP-ATG8a and npr1 (GFP-ATG8a) when plants treated with AvrRpt2 at 3 h, 6 h and 12 h and quantitative analyses of GFP/GFP-ATG8a/Actin ratio. Each data is three independent replicates. Each value is the mean ± SD of three replicates. Statistically significant differences between treatments (# p < 0.05, * p < 0.05, ** p < 0.01 and *** p < 0.001). (C) Quantitative RT-PCR data showed the expression of ATG1, ATG6 and ATG8a in wild-type (WT) and npr1 after AvrRpt2 infiltration for 3 h, 6 h and 9 h. The CK group was treated with MgCl2 as control. Each data is three independent replicates. Each value is the mean ± SD of three replicates. Statistically significant differences between treatments (* p < 0.05, ** p < 0.01 and *** p < 0.001).