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. 2020 Jul 21;21(14):5147. doi: 10.3390/ijms21145147

Figure 4.

Figure 4

ATG4 inhibited salicylic acid (SA) consumption during AvrRpt2 induced autophagy-dependent HR. (A) Total SA and free SA contents in WT and atg4a4b under AvrRpt2 infection for 12 h. (B) Quantitative RT-PCR data showed the expression of isochorismate synthase 1 (ICS1) in WT and atg4a4b when infected with AvrRpt2. (C) Quantitative RT-PCR data showed the expression of NPR1, NPR3 and NPR4 in WT and atg4a4b when infected with AvrRpt2. (D) Western Blot to detect NPR1 in WT, rps2, npr1, atg5, atg8a and atg4a4b when plants treated with AvrRpt2 for 12 h and quantitative analyses of the results of NPR1 by statistical methods. The NPR1 level was determined on the basis of the ratio of the NPR1 band intensity to that of the non-specific band (asterisk). Each value is the mean ± SD of three replicates. Statistically significant differences between treatments (* p < 0.05, ** p < 0.01 and *** p < 0.001).