Figure 10.
Induction of LC3 puncta by AuNP treatment. A) Formation of cyan fluorescent protein (CFP)‐tagged LC3 (LC3‐CFP) (pseudocolored as green) in CFP‐LC3 normal rat kidney (NRK) cells treated for 24 h with 1 × 10−9 m AuNPs. Left, confocal image; right, bright‐field image (scale bar, 10 µm). B) Statistical analysis of the number of autophagosomes per cell after 24 h of treatment. C) Conversion of LC3 from the cytoplasmic form (LC‐I) to the autophagosome‐associated form (LC3‐II). Adapted with permission.[ 197 ] Copyright 2011, American Chemical Society.