Table 2.
List of the antibodies used for flow cytometry.
| Target | Host/Clone | Fluorochrome | Supplier, Cat. Number | Dilution in the Assay |
|---|---|---|---|---|
| pERK1/2 (Thr202/Tyr204) | Mouse mAb, 4B11B69 | Alexa Fluor® 488 | Biolegend, 675508 | 1:25 |
| Bcl-xl | Rabbit mAb, 54H6 | Alexa Fluor® 488 | Cell Sign. Techn. 2767 |
1:75 |
| pAkt (Ser473) | Rabbit mAb, D9E | Alexa Fluor® 488 | Cell Sign. Techn. 4071 |
1:50 |
| Caspase-3 (Asp175) | Rabbit polyclonal | none | Cell Sign. Techn. 9661 |
1:500 |
| Anti-rabbit (H+L) | Goat polyclonal | Alexa Fluor® 488 | Thermo Fisher Sci. A-11008 |
1:600 |
| CD11b | Mouse mAb, LT11 | FITC | ImmunoTools, 21389113 | 1:20 |
| CD33 | Mouse mAb, WM53 | PE-Cy5.5 | Thermo Fisher Sci. A15454 |
1:00 |
| CD45 | Mouse mAb, 30-F11 | FITC | Biolegend, 103107 | 1:200 |
| CD11b | Rat mAb, M1/70 | PE | Biolegend, 101208 | 1:80 |
| Ly6C | Rat mAb, HK1.4 | APC | Biolegend, 128016 | 1:80 |
| Ly6G | Rat mAb, 1A8 | APC | Biolegend, 127614 | 1:330 |