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. 2020 Jul 21;21(14):5151. doi: 10.3390/ijms21145151

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Estimation of direct miRNA-target interaction by luciferase reporter assay. GIST-T1 cells were cotransfected with miRNA mimic (or miRNA mimic negative control) and pmiR-REPORT luciferase vector, containing wild-type (wt) or mutant (mut) 3′UTR sequences of the predicted target genes. Several different miRNA binding positions (pos) in the predicted target gene were investigated (Tables S1 and S2). Luciferase activity was normalized to the β-galactosidase signals. Results are shown as a percentage relative to the mimic negative control (miR-NC). Data from three to five independent experiments. * p < 0.05.