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. 2020 Jul 7;12(31):34610–34619. doi: 10.1021/acsami.0c08592

Figure 5.

Figure 5

Growth of HGF cells on NIR-irradiated (1 W/cm2, 808 nm), PDA-NP-coated (200 μg/cm2) titanium surfaces in a per-operative contamination model. (A) Schematics of bicultures for mimicking per-operative contamination (A1), in which the implant surface is contaminated with S. aureus ATCC12600 (1 × 103 CFU/cm2) and irradiated before tissue integration by HGFs. Fluorescence images (A2) represent DAPI/TRITC-stained HGF cells grown for 24 h on S. aureus-contaminated, PDA-NP-coated titanium surfaces, in the absence (0 min) and presence of 3 min irradiation (samples immersed in 10 μL of DMEM-HG medium). Red fluorescence indicates skeleton spreading, and blue fluorescence indicates HGF nuclei. The scale bar represents 100 μm. (B) Surface coverage by adhering HGFs on bacteria-contaminated, PDA-NP-coated titanium surfaces in the absence (0 min) and presence of irradiation while immersed in different DMEM-HG volumes. (C) Same as (B) but for the number of adhering HGFs per unit area. Error bars denote SEM over three experiments with separately cultured cells. * Denotes a significant improvement, i.e., a significant decrease upon NIR irradiation (p < 0.05), compared with staphylococcal contamination in the absence of NIR irradiation. # Denotes similarity, i.e., no significant difference in the presence of staphylococcal contamination and after NIR irradiation (p > 0.05), compared with the absence of staphylococcal contamination.