Figure 1.

hsf-1 and affl-2 were discovered in a screen for suppressors of heat-shock-induced lin-3c overexpression. (A) Outline of screening process. The F₂ progeny of mutagenized Phsp-16.41:lin3c animals were screened for suppression of lin-3c overexpression induced pumping quiescence. Gene diagrams of hsf-1 (B) and affl-2 (C). Blocks are exons, lines are introns, and red arrows indicate position of molecular changes for alleles. (B) Purple indicates the DNA-binding domain of HSF-1, and the red box represents the transactivation domain. Sequences for alleles can be found in Table 1. (D) Annotated AFFL-2 sequence. Highlighted text in yellow corresponds to predicted NLSs (see Materials and Methods), and disrupted residues due to allele change are underlined in red text. * indicates nonsense mutation; # indicates splice site disruption prior to residue; ∼ indicates first disrupted residue of missense mutation and frameshift caused by sy509.