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. 2020 Jun 9;215(4):1039–1054. doi: 10.1534/genetics.120.302923

Figure 6.

Figure 6

Analysis of the interaction of hsf-1, affl-2, and affl-1. All genotypes contain drSi13, which is the single-copy transgene with Phsf-1::HSF-1::GFP. (A) Representative images of hypodermis nuclei in young adults. Subcellular localization of HSF-1::GFP with and without a 5-min heat shock at 35°. Prior to heat shock, HSF-1::GFP is distributed throughout the nucleus for all genotypes; after heat shock HSF-1::GFP forms nuclear granules. Images of nuclei are from the hypodermis but are representative of HSF-1::GFP localization throughout the entire animal. In top row, representative nuclei are circled. In bottom row, examples of nuclear granules are indicated with arrows. (B) Quantification of HSF-1::GFP granules per nucleus after heat shock for different genotypes. Each point represents one nucleus. Box plots represent the 25th percentile (bottom of box), median (center line of box), and 75th percentile (top of box) of the data. Data are from 15 N2, 19 affl-2(sy975), 13 affl-1(sy1202), and 21 affl-2(sy975) affl-1(sy1220) animals.