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. 2020 Jun 8;215(4):903–921. doi: 10.1534/genetics.120.303388

Figure 4.

Figure 4

Quantification of bipartite reporter expression. Quantification of GFP intensity in PLM soma, ALM soma, and ALM axon of various combinations of reporter and driver RMCE integration events. Quantification was performed using widefield images acquired with a 40× air lens. Individual measurements (circles) and the mean (line) are shown. Arbitrary units (A.U.) are defined identically for all measurements. The data for each strain analyzed is presented in a separate row of the figure. The position and type of reporter and driver locus in each strain is shown on the left. In bold at the top of each column is the RMCE integration site (site I = jsTi1453, site II = jsTi1492, site IVa = jsTi1493, site IVb = jsTi1490), and in the column is an abbreviated description of the specific integrated reporter and driver present in the strain. UAS, QUAS, lexO, and tetO refer to reporters using these binding sites in combination with ∆pes-10 GFP-C1 tbb-2 3′. The tetO derivatives with the alternate act-4 3′ UTR, or alternate basal promoters are labeled. lexA, tetR, and rtetR driver names are shorted and lack the “-L-QFAD” designations. Statistics (n, mean, SE, and P-values of comparisons) in Supplemental Materials. Complete genotypes: (1) jsTi1453 jsSi1514 [mec-4p GFP-C1 tbb-2 3′] I, (2) jsTi1453 jsSi1518 [UAS 11X GFP-C1 tbb-2 3′] I; jsTi1493 jsSi1515 [mec-4p GAL4-QFADtbb-2 3′] IV. (3) jsTi1492 jsSi1552 [UAS 11X GFP-C1 tbb-2 3′] II; jsTi1493 jsSi1515 [mec-4p GAL4-QFADtbb-2 3′] IV. (4) jsTi1453 jsSi1518 [UAS 11X GFP-C1 tbb-2 3′] I; jsTi1490 jsIs1528 [mec-4p GAL4-QFADtbb-2 3′] IV. (5) jsTi1493 jsSi1515 [mec-4p GAL4-QFADtbb-2 3′] IV; syIs390 [UAS 15X Δpes-10 GFP let-858 3′; ttx-3p RFP]. (6) jsTi1453 jsSi1517 [QUAS 5X GFP-C1 tbb-2 3′] I; jsTi1493 jsSi1551 [mec-4p QF act-4 3′] IV. (7) jsTi1492 jsSi1504 [QUAS 5X GFP-C1 tbb-2 3′] II; jsTi1493 jsSi1551 [mec-4p QF act-4 3′] IV. (8) jsTi1453 jsSi1517 [QUAS 5X GFP-C1 tbb-2 3′] I; jsTi1493 jsSi1554 [mec-4p QF2 act-4 3′] IV. (9) jsTi1492 jsSi1504 [QUAS 5X GFP-C1 tbb-2 3′] II; jsTi1493 jsSi1554 [mec-4p QF2 act-4 3′] IV. (10) jsTi1453 jsSi1517 [QUAS 5X GFP-C1 tbb-2 3′] I; jsTi1490 jsSi1553 [mec-4p QF act-4 3′] IV. (11) jsTi1492 jsSi1504 [QUAS 5X GFP-C1 tbb-2 3′] II; jsTi1490 jsSi1553 [mec-4p QF act-4 3′] IV. (12) jsTi1453 jsSi1527 [lexO 5X GFP-C1 tbb-2 3′] I; jsTi1493 jsSi1549 [mec-4p lexA-L-QFADact-4 3′] IV, (13) jsTi1492 jsSi1520 [lexO 5X GFP-C1 tbb-2 3′] II; jsTi1493 jsSi1549 [mec-4p lexA-L-QFADact-4 3′] IV, (14) jsTi1453 jsSi1527 [lexO 5X GFP-C1 tbb-2 3′] I; jsTi1490 jsSi1555 [mec-4p lexA-L-QFADact-4 3′] IV, (15) jsTi1492 jsSi1520 [lexO 5X GFP-C1 tbb-2 3′] II; jsTi1490 jsSi1555 [mec-4p lexA-L-QFADact-4 3′] IV, (16) jsTi1453 jsSi1519 [tetO 7X GFP-C1 tbb-2 3′] I; jsTi1493 jsSi1548 [mec-4p rtetR-L-QFADact-4 3′] IV, (17) jsTi1492 jsSi1537 [tetO 7X GFP-C1 tbb-2 3′] II; jsTi1493 jsSi1548 [mec-4p rtetR-L-QFADact-4 3′] IV, (18) jsTi1453 jsSi1539 [tetO 7X GFP-C1 act-4 3′] I; jsTi1493 jsSi1548 [mec-4p rtetR-L-QFADact-4 3′] IV, (19) jsTi1453 jsSi1532 [tetO 7X ∆hsp-16.1 GFP-C1 tbb-2 3′] I; jsTi1493 jsSi1548 [mec-4p rtetR-L-QFADact-4 3′] IV, (20) jsTi1453 jsSi1543 [tetO 7X ∆mec-7 GFP-C1 tbb-2 3′] I; jsTi1493 jsSi1548 [mec-4p rtetR-L-QFADact-4 3′] IV, (21) jsTi1453 jsSi1544 [tetO 7X ∆myo-2 GFP-C1 tbb-2 3′] I; jsTi1493 jsSi1548 [mec-4p rtetR-L-QFADact-4 3′] IV, (22) jsTi1453 jsSi1519 [tetO 7X GFP-C1 tbb-2 3′] I; jsTi1493 jsSi1560 [mec-4p tetR-L-QFADact-4 3′] IV.