Table 1. Summary of quantified injections.
INJ # | Injecteda | F0 Plates | F1 Rolsb | F1 Plates | F1 plates with F2 Rolsc | F2 Rols | F1 plates with integrants |
---|---|---|---|---|---|---|---|
126 | 7 | 4 | 30 | 3 | 3 (1–2) | 5 | 2 |
127 | 10 | 4 | 92 | 12 | 6 (2–10+) | 36 | 5 |
131 | 10 | 3 | 91 | 16 | 11 (1–10+) | >39 | 10 |
136 | 8 | 2 | 52 | 9 | 3 (1–10+) | >17 | 3 |
141 | 12 | 5 | 34 | 7 | 1 (4) | 4 | 1 |
142 | 12 | 4 | 54 | 9 | 1 (1) | 1 | 1 |
143 | 9 | 3 | 43 | 8 | 2 (2–10+) | >20 | 2 |
144 | 3 | 1 | 10 | 2 | 0 | 1 | 0 |
145 | 10 | 4 | 107 | 18 | 7 (1–10+) | >100 | 5 |
146 | 8 | 3 | 38 | 8 | 3 (1–3) | 7 | 3 |
147 | 9 | 3 | 88 | 15 | 5 (1–4) | 13 | 4 |
148 | 7 | 3 | 67 | 13 | 7 (1–40+) | >50 | 6 |
149 | 10 | 3 | 43 | 9 | 7 (4–30+) | >50 | 7 |
150 | 6 | 3 | 42 | 7 | 1 | 2 | 1 |
151 | 12 | 4 | 54 | 10 | 4 (1–20+) | >50 | 2 |
152 | 7 | 3 | 25 | 5 | 1 | 1 | 1 |
153 | 10 | 3 | 72 | 13 | 6 (1–8) | 19 | 5 |
154 | 3 | 1 | 4 | 4 | 1 | 1 | 1 |
156 | 8 | 3 | 18 | 4 | 1 (3) | 3 | 1 |
156B | 7 | 2 | 23 | 5 | 3 (2–6+) | >15 | 3 |
157 | 12 | 4 | 104 | 18 | 6 (1–6+) | >16 | 6 |
158 | 13 | 4 | 24 | 5 | 4 (1–2) | 6 | 3 |
159 | 12 | 4 | 37 | 8 | 4 (1–20+) | >30 | 4 |
160 | 9 | 3 | 46 | 11 | 1 | 1 | 1 |
161 | 9 | 3 | 24 | 6 | 1 (5) | 5 | 1 |
162 | 12 | 4 | 55 | 12 | 0 | 0 | 0 |
163 | 10 | 4 | 143 | 24 | 7 (1–10+) | >25 | 7 |
166 | 10 | 4 | 21 | 6 | 1 | 1 | 1 |
169 | 8 | 2 | 29 | 5 | 5 (1–30+) | >32 | 4 |
170 | 9 | 3 | 55 | 9 | 3 | 3 | 2 |
SUMMARY | 272 | 96 | 1525 | 281 | 105 (1–40+) | ≫548 | 92 |
See Table S1 for an extended version of this table that lists total animals injected, strains injected, plasmids injected, concentration of DNA and other extended analysis.
Number of well injected P0 animals. See methods for definition.
Rol animals were picked at all ages. Some animals picked at L2/L3 stage did not Rol as adults. Both adult Rol and non-Rol transmitted Rol F2 animals.
Number of independent plates of pooled F1 Rols that yielded F2 Rol animals. In parentheses is the range of Rol animals found F1 plates with Rol animals.