Figure 5.

Insulator and promoter activities of the scs’ M fragment can be separated despite overlapping. LS4 and the BEAF binding site (H) are important for both insulator and promoter function of the scs’ M fragment. The 3′ end plays a role in insulator function, but not promoter function. The 5′ end plays a role in promoter function, but not insulator function. Spacing between LS4 and H is important for insulator function while the spacer sequence is not, whereas the spacer sequence does play a role in promoter function.