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Meiotic spreads at pachytene stages. (A) Male C. elongatoides, (B) triploid ETT hybrid female, (C) diploid ET hybrid male and (D) triploid ETT hybrid male. Synaptonemal complexes visualized by immunolabeling with antibodies against SYCP3 protein (green) and SYCP1 (red), stained with DAPI (blue). Synapsed chromosomes show both SYCP3 and SYCP1 localization (indicated by arrows), while asynapsed chromosomes exhibit only SYCP3 staining (arrowheads). Bar, 10 mm.
