Figure 7.

Humanized cytoskeletal yeast strains with abnormal morphologies phenocopy deletions of their corresponding yeast or tholog’s interaction partner. (A) Schematic depicting the nature of physical/genetic interactions in wild-type (Left), humanized (Middle), and interacting partner deletion (Right) yeast strains. Humanized yeast and the deletion strains are shown to cause similar abnormal cell shapes. (B) Distribution of circularity indices of viable haploid deletion strains. Gene deletions leading to significantly elongated and circular morphologies would lie in the blue and red regions respectively. (C) Overlaps of gene deletions leading to circular morphology with ACT1 physical (left) and genetic (right) interactors. (D) Overlaps of gene deletions leading to elongated morphologies with CDC10 physical (left) and genetic (right) interactors. Cell shape parameters computed from data from SCMD. ACT1 and CDC10 interactors curated from SGD (for additional details see Table S5). P-values determined by hypergeometric test.