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. 2020 Jul 17;21(14):5054. doi: 10.3390/ijms21145054

Figure 2.

Figure 2

Absorption rate of oxygen by sperm after cultivation under simulated microgravity and hypergravity conditions. (A)—cultivation for 1 h; (B)—cultivation for 3 h; (C)—cultivation for 6 h. CS—static control, CD—dynamic control, s-μg—simulated microgravity conditions, 2g—hypergravity conditions. V0—respiration rate of permeabilized cells; Vglu+mal—respiration rate after addition of 10 mM glutamate + 5 mM malate; Vmax—maximum respiration rate after addition of 2 mM ADP; V(II)—respiration rate after addition of 0.5 mM rotenone (complex I inhibitor) and then 10 mM succinate (substrate of complex II); V(IV)—respiration rate after addition of 5 mM antimycin (complex III inhibitor) and then 0.5 mM TMPD + 2 mM ascorbate (artificial substrates of complex IV). The change in respiration rate from V0 to V(II) in increased resolution is presented on the graphs in the frame. * p < 0.05 compared with the corresponding static control group. The data are presented as the mean ± standard error of the mean (M ± SE).