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. 2020 Jul 17;21(14):5065. doi: 10.3390/ijms21145065

Figure 1.

Figure 1

DNA stabilizes tryptase activity at room temperature. (A) β-tryptase (0.7 ng/µL in PBS, pH 7.2) was incubated at room temperature either alone or in the presence of either heparin, dsDNA or ssDNA (ratio 1:5) at the time periods indicated. Residual tryptase activity was then measured using the chromogenic substrate S-2288. (B–D) Dose response experiments for β-tryptase (0.7 ng/µL; in PBS, pH 7.2) after incubation for 3 h at room temperature, in the absence or presence of either heparin (B), dsDNA (C) or ssDNA (D) at the indicated concentrations. Residual tryptase activity was then measured using the chromogenic substrate S-2288. The data are representative of at least 3 individual experiments. Data are given as mean values ± SD (n = 3) ***, p ≤ 0.001 **, p ≤ 0.01; ns, not significant (One-way ANOVA).