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. 2020 Jul 17;21(14):5065. doi: 10.3390/ijms21145065

Figure 2.

Figure 2

DNA stabilizes tryptase activity at 37 °C. (A) β-tryptase (1 ng/µL; in PBS, pH 7.2) was incubated at 37 °C either alone or in the presence of either heparin, dsDNA or ssDNA (ratio 1:5) at the time periods indicated. Residual tryptase activity was then measured using the chromogenic substrate S-2288. (B, C) Dose response experiments were performed where tryptase (1 ng/µL) was incubated for 3 h at 37 °C, in the absence or presence of either heparin, dsDNA or ssDNA at the indicated concentrations. (D) Initial reaction velocities from B–C are displayed. Residual tryptase activity was measured using the chromogenic substrate S-2288. The data are representative of at least 3 individual experiments. Data are given as mean values ± SD (n = 3) *, p ≤ 0.01; ns, not significant (One-way ANOVA).